Effect of oral bromocriptine parlodel on the endometrium of the adult female albino rat I A light microscopic study

The response of the endometrium to oral bromocriptine administration in low and high doses for 30 days and the effect of the drug withdrawal, 15 days after the last dose, was studied in adult albino rat. Both doses increased the thickness of the endometrial epithelial lining due to focal transformation into the pseudostratified columnar or even the stratified columnar types. However, the latter type was seen only in high dose-given animals. Bromocriptine administration in its low and high doses markedly increased the size of the stromal cell which contained abnormally abundant cytoplasm and oval around vesicular nuclei. These cells were separated by markedly increased collagen fiber. This was accompanied by the presence of large numbers of eosinophilis deep in the stroma. The drug increased markedly the cytoplasmic RNA content and lowered the DNA density in all endometrial cellular elements. Very highly satistical significant increase in the overall endome trial thickness occurred in response to bromocriptine administration. 15 days after the last bromocriptine dose, the endometrium regained its more less normal structure

Effects of oral bromocriptine Parlodel on the endometrium of the adult female albino rat II- an electron microscopic study

The effect of bromocriptine on the endometrial ultrastructure was studied in specimens obtained form adult rats after receiving a single daily oral dose of "Parlodel" equivalent to the human either low or high dose, for 30 consecutive days. Both doses markedly increased the thickness of the uterine epithelial lining due to focal transformation of the normal simple cuboidal or low columnar epithelium into pseudostratified or even stratified columnar epithelium. Ultrastructurally, the epithelial lining in both the control and the low dose-given animals contained only one type of epithelial cells, but only in the high dose-given animals were these cells differentiated into two types the dark and the pale cells reflecting different degree of cellular protein synthesis activity. Both the low and high doses increased the cellular content of the organelles concerned with protein synthesis. The most striking cellular ultrastructural change, in response to the low and the high doses, was the presence of intracytoplasmic non-membrane bound huge vacuoles for which the term "autolytic vacuoles" was suggested, because they started as aggregates of primary lysosomes surrounded by autolysed cytoplasm most probably due to damaged leaky lysosomal membranes. With both doses, most of the stromal cells became abnormally large with an obvious increase in the amount of cytoplasm and its content of the organelles concerned with protein synthesis, The forementioned results suggest that, at least in rats, the bromocriptine increase the endometrial cellular activity probably through an estrogenic effect

Effect of long acting bromocriptine on the endometrium of adult female albino rat II an electron microscopic study

Long acting bromocriptine injections were intramuscularly administered to adult female albino rats. Each rat received a single dose equivalent to either the low or high human therapeutic dose. Uterine specimens were collected 2 and 15 days after the injection to study the effect of the drug on the endometrium. Both doses markedly increased the thickness of the uterine epithelial lining due to focal transformation of the normal simple cuboidal or low columnar epithelium into tall columnar, pseudostratified or even stratified columnar epithelium. According to their fine structure, both the epithelial lining and the glandular epithelium in the control animals contained only one type of cells. However, 2 days after the low dose injection the cells were differentiated into 3 types, the pale, the dark and the intermediate reflecting different degrees of cellular protein synthesis activity. Two days after the high dose injection both the lining and glandular epithelia were transformed into the pseudostratified columnar and tall columnar type, respectively

Effect of oral bromocriptine on the endometrial structure of adult female albino rat

The response of the endometrium to oral bromocriptine administration in low and high doses for 30 days and the effect of the drug withdrawal, 15 days after the last dose, was studied in adult albino rat. Both doses increased the thickness of the endometrial epithelial lining due to focal transformation into the pseudostratified columnar or even the stratified columnar types. However, the latter type was seen only in high dose-given animals. The cytoplasm of most of the epithelial cells was vacuolated. These vacuoles were variable in shape and size and contained lightly stained eosinophilic material and deeply stained basophilic granules. Both doses increased the thickness of the glandular epithelium of some glands where the epithelium was transformed into the pseudostratified columnar type. However, some other glands were abnormally cystically dilated and lined by simple squamous epithelium. Bromocriptine administration in its low and high doses markedly increased the size of the stromal cells which contained abnormally abundant cytoplasm and oval or rounded vesicular nuclei

Effect of oral bromocriptine on the endometrial ultrastructure of adult female albino rat

The effect of bromocriptine on the endometrial ultrastructure was studied in specimens obtained from adult rats after receiving a single daily oral dose of "parlodel" equivalent to the human either low or high dose, for 30 consecutive days. Both doses markedly increased the thickness of the uterine epithelial lining due to focal transformation of the normal simple cuboidal or low columnar epithelium into pseudostratified or even stratified columnar epithelium. Ultrastructurally, the epithelial lining in both the control and the low dose-given animals contained only one type of epithelial cells, but only in the high dose-given animals these cells were differentiated into two types, the dark and the pale cells reflecting different degree of cellular protein synthesis activity. Both the low and high doses increased the cellular content of the organelles concerned with protein synthesis. The most striking cellular ultrastructural change, in response to the low and the high doses was the presence of intracytoplasmic nonmembrane bound huge vacuoles for which the term "autolytic vacuoles" was suggested because they started as aggregates of primary lysosomes surrounded by autolysed cytoplasm most probably due to damaged leaky lysosomal membranes. With both doses, most of the stromal cells became abnormally large with an obvious increase in the amount of cytoplasm and its content of the organelles concerned with protein synthesis, collagen fibers were also increased. The forementioned results suggested that, at least in rats, the bromocriptine increases the endometrial cellular activity probably through an estrogenic effect